paper n a recombinant dna plasmid pgadt7 takara  (TaKaRa)

Journal: Horticulture Research

Article Title: VvSUN may act in the auxin pathway to regulate fruit shape in grape

doi: 10.1093/hr/uhac200

Figure Lengend Snippet: Analysis of the VvSUN promoter and location analysis of VvSUN . (A) VvARF6 interfaces with the VvSUN promoter, as shown by a Y1H assay. As bait, Y1H strains containing the promoters of VvSUN were employed in the experiment. pGADT7 was set as the vector for the control. (B) Measurement of GUS expression levels in grapevine leaves was accomplished based on the transient expression of the VvSUN promoter in response to varying dosages of IAA treatment. The 35S promoter acted as the positive control. Outcomes are presented as the means (± standard deviation) of three biological experiments. Lower-case letters ( P ≤ .01) and capital letters ( P ≤ .05) represent a significant difference among different treatments, as determined by Student’s t -test. (C) Histochemical staining of GUS function mediated by the VvSUN promoter under different concentrations of IAA treatment in grapevine leaves. The 35S promoter is the control and P0, P10, P50, and P100 represent the 0, 10, 50, and 100 mg/l IAA treatments, respectively. (D) Subcellular distribution of VvSUN in the leaves of N. benthamiana that had been transiently converted. 35S-GFP was adopted as the control, and expression was monitored utilizing a confocal laser scanning microscope. Chlorophyll channel (left 1), GFP channel (left 2), bright field (right 2), and merged images (right 1) are shown.

Article Snippet: Co-transformation of recombinant plasmid pGADT7-VvARF6 and pAbAi-VvSUN-pro into yeast strain Y1HGold (Clontech) was performed in accordance with the guidelines provided by the manufacturer.

Techniques: Y1H Assay, Plasmid Preparation, Expressing, Positive Control, Standard Deviation, Staining, Laser-Scanning Microscopy

Journal: Horticulture Research

Article Title: VvSUN may act in the auxin pathway to regulate fruit shape in grape

doi: 10.1093/hr/uhac200

Figure Lengend Snippet: The pathway correlation network and proposed model of fruit shape control. (A) Key pathways in the control of fruit shape by WGCNA and KEGG analysis were characterized and identified, then significantly enriched pathways were used to construct a correlation network by Cytoscape. (B) Proposed model of how VvSUN regulates fruit shape. VvSUN is situated in the plasma membrane and exogenous auxin may induce expression of VvARF6 , then VvARF6 activates cis -elements in VvSUN promoters to induce gene expression. The increased expression of VvSUN stimulates endogenous auxin accumulation and polar transport and/or auxin signal transduction process variations. Therefore, we suppose that VvSUN may not only respond to exogenous auxin treatment but also modulate the elongated fruit shape in the plant hormone signal transduction pathway during the early phases of fruit growth.

Article Snippet: Co-transformation of recombinant plasmid pGADT7-VvARF6 and pAbAi-VvSUN-pro into yeast strain Y1HGold (Clontech) was performed in accordance with the guidelines provided by the manufacturer.

Techniques: Construct, Expressing, Transduction

Journal: eLife

Article Title: Myofibril diameter is set by a finely tuned mechanism of protein oligomerization in Drosophila

doi: 10.7554/eLife.50496

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Plasmid: pGADT7-GW , Addgene , 61702 , .

Techniques: FLAG-tag, Transgenic Assay, Plasmid Preparation, Sequencing, Synthesized, Clone Assay, CRISPR, Mutagenesis, Recombinant, Cloning, Software